Invited Talk 21st International Conference on Biological Inorganic Chemistry 2025

A very short journey exploring de novo designed mononuclear metalloproteins (#123)

Vincent Pecoraro 1 , Salvatore La Gatta 1 , emerson ducasse 1 , valerie greenwood 1 , jacob firby 1 , james E Penner-Hahn 1 , jeanne Stuckey 1
  1. University of Michigan, Ann Arbor, MI, United States

De novo designed proteins often provide a simplified scaffold by which one may interrogate metal behavior in biocatalytic systems.  We will present work that exploits three-stranded coiled coils that bind a variety of metals in a wide range of coordination environments with the intent to define precise structural environments and enhanced catalytic activities. Systems that will be explored contain cobalt(II), which may serve as a spectroscopic probe as well as a catalytic center. Different perturbations from the classical canonical coiled coil architecture will be presented and discussed from evolutionary approach to protein design.