Poster Presentation 21st International Conference on Biological Inorganic Chemistry 2025

G-quadruplex (GQ) formed by guanine-rich DNA strands exhibits superior thermal stability and electric properties, which has generated substantial interests in applying GQ DNA to bioelectric interface. However, single G-wires formed by GQs have not yet surpassed the µm length due to the lack of an optimal assembly protocol and understandings of assembly mechanisms that limits the applications. Herein, we optimized a self-assembling protocol for a short 4-nt oligonucleotide (dG4) to achieve micro-meter lengths of G-wires, including buffer composition, incubation process and surface assembly. We further developed a redox DNAzyme based upon a G-wire/heme adduct. The DNAzyme exhibits remarkable temperature-enhanced peroxidase-mimetic activity, prolonged reaction duration under high temperature and superior conversion yield. Both theoretical and chemical modification study shows a stepwise mechanism from nucleation to grow into oligomer and nanowire that promotes catalytic performance of DNAzyme under elevated temperature.