Poster Presentation 21st International Conference on Biological Inorganic Chemistry 2025

Establishing a foundation for the development of synthetic antibiotics targeting the iron-sulfur cluster biogenesis pathway of Staphylococcus aureus (#489)

Connor Nelson 1 , Emily Sabo 1 , Nupur Tyagi 2 , Veronica Stark 1 , Katelyn Aasman 1 , Ava Garrison 1 , Ashlynne Bogema 3 , Christine N Morrison 1 , Jeffrey M Boyd 2 , Richard C Holz 1
  1. Chemistry, Colorado School of Mines, Golden, COLORADO, United States
  2. Department of Biochemistry and Microbiology, Rutgers University, New Brunswick, New Jersey, United States
  3. Quantitative Biosciences and Engineering, Colorado School of Mines, Golden, Colorado, United States
Publish consent withheld
  1. (1) Hudspeth, J.D.; Boncella, A.E.; Sabo, E.T.; Andrews T.; Boyd, J.M.; Morrison, C.M. Structural and Biochemical Characterization of Staphylococcus aureus Cysteine Desulfurase Complex SufSU. ACS Omega, 2022, 7 (48), 44124–44133. DOI: https://doi.org/10.1021/acsomega.2c05576.
  2. (2) Roberts, C.A.; Al-Tameemi, H.M.; Mashruwala, A.A.; Rosario-Cruz, Z.; Chauhan, U.; Sause, W.E.; Torres, V.J.; Belden, W.J.; Boyd, J.M. The Suf Iron-Sulfur Cluster Biosynthetic System Is Essential in Staphylococcus aureus, and Decreased Suf Function Results in Global Metabolic Defects and Reduced Survival in Human Neutrophils. Infect. Immun., 2017, 85 (6), DOI: https://doi.org/10.1128/iai.00100-17.
  3. (3) Fujishiro, T.; Terahata, T.; Kunichika, K.; Yokoyama, N.; Maruyama, C.; Asai, K.; Takahashi, Y. Zinc-Ligand Swapping Mediated Complex Formation and Sulfur Transfer between SufS and SufU for Iron–Sulfur Cluster Biogenesis in Bacillus subtilis. J. Am. Chem. Soc. 2017, 139 (51), 18464–18467, DOI: https://doi.org/10.1021/jacs.7b11307.
  4. (4) Sabo, E.; Nelson, C.; Tyagi, N.; Stark, V.; Aasman, K.; Morrison, C.N.; Boyd, J.M.; Holz, R.C. Development of a Spectrophotometric Assay for the Cysteine Desulfurase from Staphylococcus aureus. Antibiotics 2025, 14 (2), 129; https://doi.org/10.3390/antibiotics14020129.